| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Avery, Anne S (12)
|
VATAC |
41.71 |
40.45 |
-1.26 |
| 2 |
Daugherty, Samantha E (12)
|
VASEVA |
43.86 |
40.58 |
-3.28 |
| 3 |
Kohler, Sofia A (12)
|
VATAC |
44.09 |
41.78 |
-2.31 |
| 4 |
Jones, Eliza A (12)
|
VACGBD |
46.32 |
43.27 |
-3.05 |
| 5 |
Torres, Arianne C (12)
|
VAWAC |
47.01 |
43.65 |
-3.36 |
| 6 |
Schuurman, Nora V (12)
|
VATAC |
|
44.01 |
- |
| 7 |
Giles, Rachel O (12)
|
VACGBD |
44.64 |
44.27 |
-0.37 |
| 8 |
O'Neil, Alexis A (12)
|
VAWAC |
47.83 |
44.93 |
-2.90 |
| 9 |
Drozda, Isabella L (12)
|
VASEVA |
51.87 |
47.59 |
-4.28 |
| 10 |
Holland, Grace S (12)
|
VACGBD |
48.76 |
48.18 |
-0.58 |
| 11 |
Dunn, Tallula M (12)
|
VA757 |
|
48.34 |
- |
| 12 |
Gonzalez, Marta V (12)
|
VAECAT |
56.22 |
49.03 |
-7.19 |
| 13 |
Woods, Marissa F (12)
|
VAPSDN |
|
49.89 |
- |
| 14 |
Berry, Madelyn J (12)
|
VAECAT |
|
50.09 |
- |
| 15 |
Andresky, Sarah L (12)
|
VA757 |
48.06 |
50.11 |
2.05 |
| 16 |
Townsend, Madison S (12)
|
VAWAC |
54.42 |
50.34 |
-4.08 |
| 17 |
Holcomb, Kathyrn J (12)
|
VASEVA |
50.30 |
50.39 |
0.09 |
| 18 |
Lee, Karen Y (12)
|
VASEVA |
52.44 |
50.43 |
-2.01 |
| 19 |
Schulz, Audrey M (12)
|
VAECAT |
|
52.43 |
- |
| 20 |
Speeks, Whitney E (12)
|
VAPSDN |
|
53.16 |
- |
| 21 |
Christopher, Ciearra R (12)
|
VASEVA |
1:03.27 |
54.45 |
-8.82 |
| 22 |
Pierce, Ayla B (12)
|
VATAC |
|
55.32 |
- |